DNA

Part:BBa_K3780029:Design

Designed by: Sumohit Sharma   Group: iGEM21_Stuttgart   (2021-10-14)


dsup optimized for E. Coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 70
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 952
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1288
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The nucleotide sequence was optimised so that the amino acid sequence fits the E. Coli expression system.



Source

The Dsup sequence is available at NCBI.

References